Journal: American Journal of Translational Research

Article Title: Probiotics enhance the effect of allergy immunotherapy on regulating antigen specific B cell activity in asthma patients

doi:

Figure Lengend Snippet: Butyrate modulates Derp1-specific B cell activity. Derp1-specific B cells were isolated from the peripheral blood of asthma patients (n = 12; before treatment). The cells were stimulated with Derp1 with or without the presence of butyrate sodium (BS; 5 mM) for 48 h. A: The cells were analyzed by ChIP. The bars indicate the STAT6 levels at the Iε promoter locus. B: The B cell extracts were analyzed by RT-qPCR. The bars indicate the mRNA levels of IgE. C: The bars indicate the HDAC1 levels at the Iε promoter locus (by ChIP). D: The bars indicate the STAT3 levels at the IL-10 promoter locus (by ChIP). E: The immune blots indicate the results of STAT6 gene knockdown. F: The bars indicate the mRNA levels of IgE and IL-10 in the B cells. G: The bars indicate the STAT6 levels at the IL-10 gene locus (by ChIP). Naive: Naive B cells. Specific: Derp1-specific B cells; the cells were treated with saline (c) or exposed to Derp1 (1 µg/ml) in the culture (d). H: The bars indicate the STAT3 reporter gene activity (by ChIP). STAT6-Oex: The cells with STAT6-overexpression. Control: The cells were transfected with an empty plasmid. The concentration of IL-6 was 20 ng/ml. The data are representatives of 3 independent experiments. *, P<0.01, compared to the medium group (compared to the naive group in panel G).

Article Snippet: STAT6 overexpression The STAT6 expressing plasmid was constructed by Genescript (Nanjing, China) and transfected to B cells with the lipofectamine reagent kit following the manufacturer’s instructions.

Techniques: Activity Assay, Isolation, Quantitative RT-PCR, Over Expression, Transfection, Plasmid Preparation, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A Novel NFIX-STAT6 Gene Fusion in Solitary Fibrous Tumor: A Case Report

doi: 10.3390/ijms22147514

Figure Lengend Snippet: Pathological characterization of solitary fibrous tumor case. ( A ) Hypercellular spindle cell neoplasm with hemangiopericytoid vascular pattern (4×); ( B ) Monotonous spindle to ovoid cells, without nuclear pleomorphism (40×); ( C ) Diffuse expression of CD34 (20×); ( D ) Heterogeneous expression of CD99 (20×); ( E ) Diffuse expression of bcl-2 (20×); ( F ) Diffuse and strong nuclear expression of STAT6 (20×) and ( G ) Ki-67 proliferative index (20×).

Article Snippet: STAT6 , Polyclonal , 1:50 , Gennova.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: A Novel NFIX-STAT6 Gene Fusion in Solitary Fibrous Tumor: A Case Report

doi: 10.3390/ijms22147514

Figure Lengend Snippet: Sanger sequencing validation of NFIX ex8 –STAT6 ex2 gene fusion.

Article Snippet: STAT6 , Polyclonal , 1:50 , Gennova.

Techniques: Sequencing, Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: A Novel NFIX-STAT6 Gene Fusion in Solitary Fibrous Tumor: A Case Report

doi: 10.3390/ijms22147514

Figure Lengend Snippet: Schematic representation of NFIX–STAT6 fusion. DNAbd: N-terminal DNA-binding domain; MH1: MAD homology 1 domain; CTF/ NFI: CAAT-box transcription factor–nuclear factor I domain; CCD1: Coiled-coil domain 1; DBD: DNA-binding domain; SH2: Src homology 2; and TAD: transcriptional activator domain.

Article Snippet: STAT6 , Polyclonal , 1:50 , Gennova.

Techniques: Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: A Novel NFIX-STAT6 Gene Fusion in Solitary Fibrous Tumor: A Case Report

doi: 10.3390/ijms22147514

Figure Lengend Snippet: Immunohistochemical reagents.

Article Snippet: STAT6 , Polyclonal , 1:50 , Gennova.

Techniques: Immunohistochemical staining